Rovers have been drawn against Swindon Town and Plymouth Argyle in the Papa Johns Trophy Southern Group E.
Rovers are joined by League Two outfit Swindon Town and fellow League One side Plymouth Argyle. One invited U21's side is set to join the group at a later date.
Last season Rovers were drawn against Exeter City, Cheltenham Town and Chelsea U21's. The Gas started well in last year's competition winning 2-0 against Cheltenham Town at The Mem. Rovers finished the group in third behind Exeter and Chelsea U21's.
The group will be completed by one of the following clubs:
Arsenal
Aston Villa
Brighton and Hove Albion
Chelsea
Crystal Palace
Southampton
Tottenham Hotspur
West Ham United