Darrell has made two changes to the side that was victorious at the Merseyrail Community Stadium on Saturday bringing Ellis Harrison and Ollie Clarke into the starting 11.
Dave Martin and Adam Cunnington drop to the substitutes bench as the boss thinks pace will hurt the Eastleigh back line.
The last time Rovers and Eastleigh met was in the FA Cup back in October in 1899.
Rovers came out on top in that game with a 5-0 win thanks to goals from Jack Jones, George Kinsey and a second half Jack Lewis hat-trick.
Rovers: Steve Mildenhall, Tom Lockyer, Neal Trotman, Tom Parkes, Lee Brown, Ollie Clarke, Lee Mansell, Stuart Sinclair, Jake Gosling, Ellis Harrison, Matty Taylor.
Substitutes: Will Puddy, Mark McChrystal, Dave Martin, Adam Cunnington, Jamie White.