Santos and Richards start
John Ward has made two changes to the side that lost to Accrington Stanley on Tuesday.
Eliot Richards and Alefe Santos take places in the starting XI, with Alex Henshall and Ellis Harrison dropping to the bench.
Bristol Rovers: Steve Mildenhall, Michael Smith, Tom Parkes, Will Packwood, Tom Lockyer, Ollie Clarke, John-Joe O'Toole, Seanan Clucas, Alefe Santos, Ellis Harrison, Ryan Brunt.
Substitutes: Conor Gough, Oliver Norburn, Pat Keary, Mitch Harding, Josh Southway, Ellis Harrison, Alex Henshall.
Chesterfield: Tommy Lee, Tendayi Darwika, Ian Evatt, Liam Cooper, Ritchie Humphries, Jamie Devitt, Sam Morsy, Jame Ryan, Eoin Doyle, Gary Roberts, Armand Gnanduillet
Substitutes: Aaron Chapman, Oliver Banks, Nathan Smith, Sam Hird, Marc Richards, Ryan Edwards, Gary McShefferey